Fig. 11.

Correlations between LPS-induced NLRP3 expression and measures of sickness-like behaviors in male C57BL/6J mice. Micro-osmotic pumps containing saline or β-FNA (42 μg/d) were surgically implanted and dispensed at a flow rate of 0.5 μL/h for 7d. 6d post-surgery, mice (n = 7–8/group) were injected (i.p.) with either 25 μL saline control or LPS (0.83 mg/kg). Behavioral tests were administered 24 h later, and termination followed immediately after. NLRP3 was measured via RT-qPCR using frontal cortex and spleen RNA extracts. Linear regression analysis was used to assess (A) frontal cortex and (B) spleen NLRP3 expression with various anxiety-, sickness-, and depressive-like behavioral endpoints. Data are presented as mean ± SEM. Linear regression statistics and symbols are provided in figure, and only significant results are shown