Fig. 2.

Chronic effects of β-FNA on LPS-induced depressive-like behavior in male C57BL/J6 mice. Micro-osmotic pumps containing saline or β-FNA (42 μg/d) were surgically implanted and dispensed at a flow rate of 0.5 μL/h for 7d. 6d post-surgery, mice (n = 7–8/group) were injected (i.p.) with either 25 μL saline control or LPS (0.83 mg/kg). Behavioral tests were administered 24 h later, and termination followed immediately after. Endpoints measured included A percentage of time spent immobile and (B) latency to immobility. Data are presented as mean ± SEM. A Two-way ANOVA indicated no significant main effect of LPS (p = 0.46), β-FNA (p = 0.20), or interaction of main effects (p = 0.74) on percentage of time immobile (n = 5–7/group). B Two-way ANOVA indicated no significant main effect of LPS (p = 0.17), β-FNA (p = 0.18) or interaction of main effects (p = 0.08) on latency to immobility (n = 4–7/group). Pairwise comparisons were assessed using Fisher's LSD test; bars with letters in common indicate data are not significantly different (p > 0.05)