Fig. 6.

Correlations between LPS-induced CCL2 levels in male C57BL/6J mouse tissues and sickness-like behavior. Micro-osmotic pumps containing saline or β-FNA (42 μg/d) were surgically implanted and dispensed at a flow rate of 0.5 μL/h for 7d. 6d post-surgery, mice (n = 7–8/group) were injected (i.p.) with either 25 μL saline control or LPS (0.83 mg/kg). Behavioral tests were administered 24 h later, and termination followed immediately after. CCL2 was measured via ELISA in brain region and spleen homogenates. Behavioral endpoints that were measured included (A) time spent motionless, (B) time spent in contracted position, and (C) total distance moved. Data are presented as mean ± SEM. Linear regression analysis was used to assess frontal cortex, hippocampus, and spleen CCL2 levels with EPM behavioral endpoints. Linear regression statistics and symbols are provided in figure, and only significant results are shown