Fig. 3.

AZD1480 Inhibits PFF-induced STAT3 Phosphorylation in the Midbrain. A. Male Line 61 mice (12–15 weeks old) were bilaterally injected in the striatum with monomer (10 µg) or PFF (10 µg). Two weeks after PFF injection, VH or AZD1480 (25 mg/kg/day) was administered by oral gavage for an additional four weeks. Lysates were obtained from mononuclear cells isolated from the midbrains of monomer (n = 3), PFF + VH (n = 5), or PFF + AZD1480 (n = 5) mice at six weeks and immunoblotted with the indicated antibodies. B. Fold-induction of p-STAT3 and total STAT3 was calculated by normalizing to GAPDH; and the ratio of p-STAT3 to total STAT3 using ImageJ 1.53t. C. Fold-induction of p-STAT1 and total STAT1 was calculated by normalizing to GAPDH; and the ratio of p-STAT1 to total STAT1 using ImageJ 1.53t. D. Quantification of p-STAT3 levels in the SNpc (4 sections/sample) by immunofluorescence staining in monomer (n = 3), PFF + VH (n = 5), or PFF + AZD1480 (n = 5) mice six weeks post injection. Statistical significance was determined by ordinary one-way ANOVA. *p < 0.05, **p < 0.01