Fig. 1.

Isolation and culture of primary hepatocytes of grass carp. Freshly digested grass carp primary hepatocytes (100 ×) (A) and (400 ×) (B). Primary hepatocytes of grass carp grown for 24 h (100 ×) (C) and (400 ×) (D). Grass carp primary hepatocytes (100 ×) grown for 48 h (E) and (400 ×) (F). Growth curve (G). ALB contents (H) of primary hepatocytes of grass carp for growing different times (24, 48, 72, 96, 120, 144 h). The results were expressed as mean ± SD of 6 independent observations. ^a–dValues having different letters are significantly different (P < 0.05). ALB, albumin