Fig. 3.

Effect of MD on primary hepatocyte death of grass carp. Observation of primary hepatocytes of grass carp by TEM: MD group (1,200 ×) (A) and (5,000 ×) (B); control group (1,200 ×) (C) and (5,000 ×) (D); Relative protein expression of Bax, Bcl-2, p62, LC3 II, cleaved-IL-1β, cleaved-caspase-1, RIP1, RIP3 (E) and quantification (F) after with or without 1 mmol/L Met treatment for 48 h. The results were expressed as mean ± SD of 3 independent observations. ^*P < 0.05, n.s: no significance. Met, methionine; N, nucleus; M, mitochondrion; LD, lipid droplet; Bax, B-cell lymphoma protein 2; Bcl-2, B-cell lymphoma-2; p62, sequestosome 1; LC3, microtubule-associated protein 1 light chain 3; IL-1β, interleukin-1β; CASP-1, cysteinyl aspartate specific proteinase-1; RIP1, receptor-interacting protein kinase 1; RIP3, receptor-interacting protein kinase 3