Fig. 5.

Effect of Met on pyroptosis of primary hepatocytes of grass carp. Gene expression of NLRP3, ASC, caspase-1, GSDME, IL-1β (A); relative protein expression of NLRP3, cleaved-caspase-1, GSDME, cleaved-IL-1β (B) and quantification (C) after with or without 1 mmol/L Met treatment for 48 h followed by 100 ng/mL lipopolysaccharide (LPS) treatment for 4 h and then 0.5 μmol/L Nig treatment for 0.5 h. The results were expressed as mean ± SD of 3 or 6 independent observations (WB: n = 3). ^*P < 0.05, ^**P < 0.01, ^***P < 0.001, n.s: no significance. Met, methionine; Nig, nigericin sodium salt; NLRP3, NOD-like receptor thermal protein domain associated protein 3; ASC, apoptosis-associated speck-like protein containing a CARD; CASP-1, cysteinyl aspartate specific proteinase-1; GSDME, gasdermin E; IL-1β, interleukin-1β