Fig. 6.

MD inhibited pyroptosis of primary hepatocytes of grass carp through increasing autophagy. Relative protein expression of LC3 II and p62 (A) and quantification (B) after with or without 1 mmol/L Met and CQ co-treatment for 48 h; relative protein expression of NLRP3, cleaved-caspase-1, cleaved-IL-1β (C) and quantification (D) after with or without 1 mmol/L Met and 20 μmol/L CQ co-treatment for 48 h followed by 100 ng/mL LPS treatment for 4 h and then 0.5 μmol/L Nig treatment for 0.5 h. The results were expressed as mean ± SD of 3 independent observations. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001, n.s: no significance. Met, methionine; CQ, chloroquine; LC3, microtubule-associated protein 1 light chain 3; p62, sequestosome 1; LPS, lipopolysaccharide; Nig, nigericin sodium salt; NLRP3, NOD-like receptor thermal protein domain associated protein 3; CASP-1, cysteinyl aspartate specific proteinase-1; IL-1β, interleukin-1β