Table 1.

Real-time PCR primer sequences

Target gene	Primer sequence forward (5′→3′)	Primer sequence reverse (5′→3′)	Accession number
NLRP3	CAGCGGCGGCCAATC	TCCGCGTACCTCCGTGAA	MW767970
ASC	TGGCGCGGGTCCTGTA	TCCGCTGCCAGTTCATGAC	MW767971
Caspase-1	ACGTCTTGCCCTGCTTATCAAC	CGCCCCTCTCCTGGTCATA	KX231773
GSDME	GCTTTTGTGCACTGGCTGACT	GAATTTTCCTCAACAGAAGCAGGAT	MT513755
IL-1β	AGAGTTTGGTGAAGAAGAGG	TTATTGTGGTTACGCTGGA	JQ692172
β-actin	GGCTGTGCTGTCCCTGTA	GGGCATAACCCTCGTAGAT	M25013

NLRP3 NOD-like receptor thermal protein domain associated protein 3, ASC Apoptosis-associated speck-like protein containing a CARD, Caspase-1 Cysteinyl aspartate specific proteinase-1, GSDME Gsdermin E, IL-1β Interleukin-1β