Figure 1.

ED-71 ameliorates bone loss in STZ-induced T2DM mice in vivo. (A), representative 3D micro-CT images of the femur in different groups, bar = 300μm. (B), HE stained images of mouse tibia (n = 5), bar = 200μm or 100μm. Red arrows indicates the position of the LD. (C), Masson stained images of mouse tibia (n = 5), bar = 100μm. (D), quantitative analysis of bone morphology including bone/tissue volume (BV/TV), trabecular bone number (Tb.N), trabecular bone thickness (Tb.Th) and trabecular separation (Tb.Sp), was performed in different groups using tibial HE staining. (E), differences in blood glucose in WT, T2DM, and T2DM + ED-71 groups at 6 weeks of treatment (n = 8). Data are expressed as mean ± SD, * p < 0.05, ** p < 0.01, *** p < 0.001.

Abbreviations: GP, Growth Plate; TB, trabecular bone; BM, bone marrow; LD, lipid droplets.