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. Author manuscript; available in PMC: 2025 Oct 1.
Published in final edited form as: Biochim Biophys Acta Mol Cell Res. 2024 Aug 17;1871(7):119817. doi: 10.1016/j.bbamcr.2024.119817

Figure 1. ADF increases Lin−EpCAMhi cell proportion and decreases cellular senescence within the SMGs of NOD mice.

Figure 1.

10-week-old female NOD mice were subjected to alternate-day fasting (ADF) or fed with standard chow ad libitum (AL) for 3 weeks. (A) Representative FACS gating strategy for Lin−EpCAMhi cell population analyses (left panel). Mean percentage of Lin−EpCAMhi population among the total SMG cells or Lin− cells in the SMGs (n = 4 - 5) (right panel). (B) Immunohistochemical staining of SMG sections for p16INK4a from female BALB/c and NOD mice aged 13 weeks (Scale bar = 100 μm). Bar graph shows the average percentage of positively stained area (n = 4). (C) Immunohistochemical staining of SMG sections for p16INK4a from NOD mice in ADF or AL group (Scale bar = 100 μm). Bar graph shows the average percentage of positively stained area (n = 9).