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. Author manuscript; available in PMC: 2025 Oct 1.
Published in final edited form as: Biochim Biophys Acta Mol Cell Res. 2024 Aug 17;1871(7):119817. doi: 10.1016/j.bbamcr.2024.119817

Figure 4. ADF alters the transcriptomic profile of Lin−EpCAMhi cells in the SMGs.

Figure 4.

Lin−EpCAMhi cells were electronically sorted from the SMGs of NOD mice that were subjected to ADF for a total of 3 weeks or fed standard chow AL. RNA-sequencing was performed, followed by bioinformatic analyses. (A) Volcano plot of the differentially expressed genes (DEGs). DEGs upregulated and downregulated by ADF are colored in red and blue, respectively (adjusted P value < 0.05 and absolute log2 fold change > 1). (B) Heatmap of hierarchical clustering analysis of the DEGs. Each row represents a single gene, and each column represents a sample. The colors indicate high (red), low (blue) and intermediate (white) expression values of the genes. (C) Significantly enriched KEGG pathways of the downregulated DEGs by fasting (FDR < 0.05). (D) Significantly enriched GO terms of the DEGs upregulated or downregulated by ADF (* FDR < 0.1; ** FDR < 0.05). (n = 2 - 3).