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. 2024 Sep 2;4(9):2295–2307. doi: 10.1158/2767-9764.CRC-24-0069

Figure 3.

Figure 3

Mybl2 supports tumoral proliferation and promotes self-renewal in prostate cancer cell line models. A, Normalized gene expression of MYBL2 in human prostate cancer cell lines. DEMETER gene dependency of MYB family members across human prostate cancer cell lines indicating MYBL2 as a significant dependency compared with other family members. B, Schematic representation of the DKO Ctl and DKO Mybl2 KO and PPKO Ctl and PPKO Mybl2 KO cell line generation using the two-step CRISPR/Cas9 method. Western blot indicating Mybl2 protein levels in the DKO Ctl, DKO Mybl2 KO, PPKO Ctl, and PPKO Mybl2 KO generated cell. C, Growth kinetics representation using relative luminescence intensity of DKO Clt vs. DKO Mybl2 KO spheroids and PPKO Ctl vs. PPKO Mybl2 KO spheroids cultured from day 0 (D0) to day 5 (D5) in a low-attachment round-bottom 96-well plate. D, Spheroid diameter and pictures of the DKO vs. DKO Mybl2 KO and PPKO vs. PPKO Mybl2 KO cells for 21 days postseeding of 500 or 350 cells/well, respectively. E, Flow cytometry analysis of the EdU-positive cells in DKO vs. DKO Mybl2 KO and PPKO vs. PPKO Mybl2 KO cells after 2 hours of EdU labeling in vitro.