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. 2024 Sep 2;13:RP94245. doi: 10.7554/eLife.94245

Key resources table.

Reagent type (species) or resource Designation Source or reference Identifiers Additional information
Strain, strain background (Synechocystis sp. PCC6803) Wildtype https://doi.org/10.1016/0076-6879(88)67088-1 GT
Strain, strain background (Synechocystis sp. PCC6803) sigE::KmR https://doi.org/10.1074/jbc.M505043200 G50
Strain, strain background (Synechocystis sp. PCC6803) SigA-8His-KmR https://doi.org/10.1111/tpj.15687 KR93
Strain, strain background (Synechocystis sp. PCC6803) SigA-3FLAG-KmR https://doi.org/10.1111/tpj.15687 KR94
Strain, strain background (Synechocystis sp. PCC6803) cyabrb2::KmR In this study KR340 The genome of GT strain was manipulated by the transformation of the plasmid VK203
Strain, strain background (Synechocystis sp. PCC6803) cyAbrB(sll0359)–3xFLAG-KmR In this study KR338 The genome of GT strain was manipulated by the transformation of the plasmid VK200
Strain, strain background (Synechocystis sp. PCC6803) cyAbrB2(sll0822)–3xFLAG-KmR In this study KR339 The genome of GT strain was manipulated by the transformation of the plasmid VK201
Strain, strain background (Synechocystis sp. PCC6803) cyabrB2::KmR ∆sigE::CmR In this study KR359 The genome of G50 strain was manipulated by the transformation of the plasmid VK82
Recombinant DNA reagent sigE∆CmR In this study VK82 Plasmid backbone:pTA2 (Toyobo), available upon request
Recombinant DNA reagent AbrB1-3F-KmR In this study VK200 Plasmid backbone:pTA2 (Toyobo), available upon request
Recombinant DNA reagent AbrB2-3F-KmR In this study VK201 Plasmid backbone:pTA2 (Toyobo), available upon request
Recombinant DNA reagent cyabrB2∆KmR In this study VK203 Plasmid backbone:pTA2 (Toyobo), available upon request
Antibody Anti-FLAG Sigma-aldrich F1804 RRID:AB_262044
For immunoprecipitation
Antibody Anti-FLAG (alkaline phosphatase conjugated) Sigma-aldrich A9469 RRID:AB_439699
For western blot (1:20,000)