Figure 3. BRC-1 is required for long noncrossover gene conversion.

(A–B) Plots of conversion tracts sequenced from recombinant ICR assay loci. Vertical grey lines indicate the positions of polymorphisms in the ICR assay with bp measurements given 3’ relative to the site of Mos1 excision (Toraason et al., 2021b; Toraason et al., 2021a;). Each horizontal line represents a single recombinant sequenced, ordered from smallest tract to largest tract within the interhomolog and non-interhomolog windows. Filled in points represent fully converted polymorphisms, while points with white interiors represent heteroduplex DNA sequences identified in conversion tracts. Tracts containing heteroduplex are marked with asterisks. High opacity horizontal lines within plots represent the minimum conversion tract length, or the distance from the most proximal to the most distal converted polymorphisms. Low opacity horizontal lines indicate the maximum conversion tract, extending from the most distal converted polymorphism to its most proximal unconverted polymorphism. Tracts from noncrossover recombinants are displayed in A, while tracts from crossover recombinants are displayed in (B). (C–D) Frequency of short noncrossover tracts (C, minimum tract length 1 bp converted at only the 12 bp polymorphism) or short crossover tracts (D, minimum tract length 198 bp) as a proportion of all tracts identified from progeny laid within the interhomolog and non-interhomolog windows. Error bars represent the 95% binomial confidence intervals of these proportions and p values were calculated using Fisher’s Exact Test. Diagrams above bar plots depict the sizes of tracts considered ‘long’ or ‘short’ in each respective group. In all panels, dashed grey lines delineate between the interhomolog window (22–58 hr post heat shock) and non-interhomolog window (10–22 hr post heat shock) timepoints.