| Gene (C. elegans) |
brc-1
|
https://wormbase.org/species/c_elegans/gene/WBGene00000264
|
WormBase ID: WBGene00000264 |
|
| Gene (C. elegans) |
brd-1
|
https://wormbase.org/species/c_elegans/gene/WBGene00000265
|
WormBase ID: WBGene00000265 |
|
| Gene (C. elegans) |
smc-5
|
https://wormbase.org/species/c_elegans/gene/WBGene00016151
|
WormBase ID: WBGene00016151 |
|
| Gene (C. elegans) |
smc-6
|
https://wormbase.org/species/c_elegans/gene/WBGene00010056
|
WormBase ID: WBGene00010056 |
|
| Gene (C. elegans) |
polq-1
|
https://wormbase.org/species/c_elegans/gene/WBGene00020964
|
WormBase ID: WBGene00020964 |
|
| Gene (C. elegans) |
lig-4
|
https://wormbase.org/species/c_elegans/gene/WBGene00002986
|
WormBase ID: WBGene00002986 |
|
| Gene (C. elegans) |
unc-5
|
https://wormbase.org/species/c_elegans/gene/WBGene00006745
|
WormBase ID: WBGene00006745 |
|
| Gene (C. elegans) |
rad-51
|
https://wormbase.org/species/c_elegans/gene/WBGene00004297
|
WormBase ID: WBGene00004297 |
|
| Strain, strain background (C. elegans) |
For C. elegans alleles and strain information, see strain list in Methods (C. elegans strains and maintenance) |
This paper |
|
See strain list in Methods (C. elegans strains and maintenance) |
| Genetic reagent (C. elegans) |
Details on C. elegans CRISPR/Cas9 editing are detailed in the Methods (CRISPR/Cas9 genome editing) |
This paper |
|
CRISPR/Cas9 transgenics were generated by InVivo Biosystems |
| Antibody |
Anti-RAD-51 (chicken polyclonal) |
Kurhanewicz et al., 2020
|
|
IF (1:1000) |
| Antibody |
Anti-RAD-51 (rabbit polyclonal) |
Yokoo et al., 2012
|
|
IF (1:5000) |
| Antibody |
Anti-mini-AID M214-3 (mouse monoclonal) |
MBL International |
|
IF (1:500) |
| Antibody |
Anti-HTP-3 (rat polyclonal) |
This study |
|
IF (1:1000) |
| Antibody |
Anti-GFP (rabbit polyclonal) |
Yokoo et al., 2012
|
|
IF (1:500) |
| Antibody |
Alexa Fluor 488 anti-rabbit (goat polyclonal) |
Thermo Fisher |
Cat# A21428 |
IF (1:200) |
| Antibody |
Alexa Fluor 488 anti-mouse (goat polyclonal) |
Thermo Fisher |
Cat# A11001 |
IF (1:200) |
| Antibody |
Alexa Fluor 555 anti-rat (goat polyclonal) |
Thermo Fisher |
Cat# A48263 |
IF (1:200) |
| Antibody |
Alexa Fluor 647 anti-chicken (goat polyclonal) |
Thermo Fisher |
Cat# A21449 |
IF (1:200) |
| Sequence-based reagent |
PCR primers for amplifying ICR and IH assay recombinants are described in Methods (Sequencing and analysis of ICR assay conversion tracts, Interhomolog assay (IH assay)) |
Toraason et al., 2021c, This Paper |
PCR Primers |
See Methods (Sequencing and analysis of ICR assay conversion tracts, Interhomolog assay (IH assay)) |
| Chemical compound, drug |
Naphthaleneacetic acid (auxin) |
PhytoTechnology Laboratories |
Cat# N610 |
10 mM |
| Software, algorithm |
Whole Gonad Analysis (R script) v1.0 |
Toraason et al., 2021a; Toraason et al., 2021d
|
https://github.com/libudalab/Gonad-Analysis-Pipeline
|
|
| Software, algorithm |
RStan |
Stan Development Team |
https://mc-stan.org/
|
|
| Software, algorithm |
Imaris 9 |
Oxford Instruments |
https://imaris.oxinst.com/products
|
|
| Software, algorithm |
FIJI |
Schindelin et al., 2012
|
https://imagej.net/software/fiji/
|
|
| Software, algorithm |
FIJI plug in – Stitcher |
Preibisch et al., 2009
|
https://imagej.net/plugins/image-stitching
|
|
