Table 4. Summary of the simulations.

Scheme	Protein system	Membrane system	Pulling rate (m/s)	Number of replicas	Length per replica (ns)
Equilibrium simulations	No AQP0	Pure SM	N/A	5	500
		2:1 SM:Chol
		1:2 SM:Chol
	One AQP0 tetramer without EC lipids	Pure SM		5	500
		2:1 SM:Chol
		1:2 SM:Chol
	One AQP0 tetramer with EC lipids	2:1 SM:Chol		5	500
		1:2 SM:Chol
	Two AQP0 tetramers with deep cholesterol	Pure SM		10	1000
	Two AQP0 tetramer without deep cholesterol	Pure SM
Force probe	Two AQP0 tetramers with deep cholesterol	Pure SM	0.1	20 replicas for each velocity	~55
			0.02		~230
			0.004		~900
	Two AQP0 tetramers without deep cholesterol	Pure SM	0.1	20 replicas for each velocity	~55
			0.02		~230
			0.004		~900