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| Gene | Sequence |
| OCN | F-TCACACTCCTCGCCCTATTG |
| R-GCTCCCAGCCATTGATACAG | |
| OPN | F-TCCAACGAAAGCCATGACCA |
| R-GCAGGTCCGTGGGAAAATCA | |
| GAPDH | F-CACATGGCCTCCAAGGAGTAA |
| R-TGAGGGTCTCTCTCTTCCTCTTG | |
| ALP | F-GCTGTAAGGACATCGCCTACCA |
| R-CCTGGCTTTCTCGTCACTCTCA | |
| RUNX2 | F-GGAGTGGACGAGGCAAGAGTT |
| R-GGTTCCCGAGGTCCATCTACT |
OCN; OPN; GAPDH: Glyceraldehyde 3-phosphate dehydrogenase; ALP; RUNX2
