Table 2.

Cycling mode of all tested genes according to the melting temperature of their primers [23].

Step	Incubation		Cycles
	Temperature (°C)	Time
UDG activation	50	2 min	Hold
Dual-lock DNA polymerase	95	2 min	Hold
Denature	95	15 s	40
Anneal	55–60	15 s
Extend	72	1 min