Figure 1.

Representative image of the gating strategy of general cell types of the kidney. Cells were labeled using the general kidney cell antibody panel. Multiplets and debris were removed by gating forward scatter area (FSC-A) against forward scatter height (FSC-H). Live cells were determined as those negative for aqua live/dead stain. Leukocytes were identified as the CD45⁺ population. The CD45⁻ population was then gated for CD31 to identify endothelial cells (CD31⁺). The CD31⁻ population was divided into quarters testing for smooth muscle (CD39⁺) and epithelial cell populations (epithelial cell adhesion molecule, EpCAM⁺). The feeder cell stain (fibroblasts and mural cells) and CD146 stain (pericytes) divided the EpCAM⁻/CD39⁻ population. Finally, podocytes (nephrin⁺) were gated for using the Feeder⁻/CD146⁻ population.