Figure 2.

Representative image of the gating strategy for epithelial subtypes. Cells were labeled using the epithelial cell subtype antibody panel. Multiplets and debris were removed by gating forward scatter area (FSC-A) against forward scatter height (FSC-H). Using the epithelial cell adhesion molecule (EpCAM⁺) population, type A-intercalating cells were gated for using the c-kit stain. The c-kit⁻ population was then gated for type B-intercalating cells (peanut agglutinin lectin, PNA⁺/Lotus tetragonoblus lectin, LTL⁻). The PNA⁻ and LTL⁺ populations were then divided into the embigin (EMB⁻) (gated for proximal tubule cells; LTL⁺) and EMB⁺ (gated for distal tubule cells; LTL⁻/PNA⁻) populations. Finally, the LTL⁻ population gated for principal cells (L1 cell adhesion molecule, L1-CAM⁺).