Figure 3.

Represenative image of the gating strategy for leukocyte subtypes. Cells were labeled using the leukocyte subtype antibody panel. Multiplets and debris were removed by gating forward scatter area (FSC-A) against forward scatter height (FSC-H). Leukocytes (CD45⁺) were gated for myeloid (CD11b⁺) and T cell (CD3⁺) subtypes. CD11b⁺ population was further divided using Ly6C (monocytes), Ly6G (granulocytes) and F4/80 (macrophages). Low and high Ly6C⁺ populations were further divided into different populations (patrolling and proinflammatory monocytes). T cell subtypes were divided using CD4 (helper T cells) and CD8 (cytotoxic T cells) markers. B cells (B220⁺) were identified within the CD3⁻/CD11b⁻ population.