Figure 4.

Debris clearance and GentleMACS dissociation reduced renal cell yields. A−C: representative flow cytometry plots of renal single-cell suspensions (A and B) and epithelial cell (epithelial cell adhesion molecule, EpCAM⁺) counts (C) from preparations with (○) and without (△) debris clearance. D−I: mean flow cytometric data of viable cells (D; aqua live/dead⁻), leukocytes (E; CD45⁺), epithelial cells (F; EpCAM⁺), endothelial cells (G; CD31⁺), fibroblasts, mural cells (H; Feeder⁺), and smooth muscle cells (I; CD39⁺) per mg of kidney tissue following automated gentleMACS (black bars) and manual mincing (white bars) of single-cell suspensions prepared using protocol 2 (P2) and protocol 3 (P3). *Significantly (P < 0.05) different compared with debris clearance (Student's t test) or gentleMACs dissociation (two-way ANOVA with Šidák post hoc analysis). Values are means ± SE, n = 5–7 animals.