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. 2024 Mar 25;56(7):469–482. doi: 10.1152/physiolgenomics.00002.2024

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Copyright © 2024 The Authors.

Licensed under Creative Commons Attribution CC-BY 4.0. Published by the American Physiological Society.

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Figure 8. — Protocol 1 single-cell preparation protocol enriched myeloid-derived cell yields. A: representative t-SNE plots of kidney samples process using protocols 1, 2, and 3 (P1, P2, and P3, respectively). Four kidney samples were concatenated from each protocol where blue dots depict cell that express the defined marker (left). B–D: total myeloid-derived cell (CD11b⁺; B), macrophage (F4/80⁺; C), and activated monocyte (Ly6C^hi; D) populations in male and female kidneys processed using P1 (black bars), P2 (gray bars), and P3 (white bars) single-cell preparation protocols. *Significantly (P < 0.05) different between protocols (two-way ANOVA with Šidák post hoc analysis). Values are means ± SE, n = 7 or 8 animals.