Figure 9.

Protocol 2 single-cell preparation protocol reduced lymphocytes. A: representative t-SNE plots of kidney samples process using protocols 1, 2, and 3 (P1, P2, and P3, respectively). Four kidney samples were concatenated from each protocol where blue dots depict cell that express the defined marker (left). B−F: total B cells (B220⁺; B), T cells (CD3⁺; C), T helper and regulatory T cells (CD4⁺; D), cytotoxic T cells (CD8⁺; E), and double negative T cells (CD4⁻/CD8⁻; F) in male and female kidneys processed using P1 (black bars), P2 (gray bars), and P3 (white bars) single-cell preparation protocols. *Significantly (P < 0.05) different between protocols (two-way ANOVA with Šidák post hoc analysis). Values are means ± SE, n = 7 or 8 animals.