Figure 10.

Replication of single-cell preparation protocols 1 and 3 in the spleen. A−D: T cell (CD3⁺; A), T helper cell (CD4⁺; B), cytotoxic T cell (CD8⁺; C), and double-negative T cell (CD4^-/CD8^-; D) counts were quantified per mg of spleen tissue from female mice via flow cytometry using three digestion methods: undigested (gray bars), protocol 1 (black bars), and protocol 3 (white bars). *Significantly (P < 0.05) different between protocols (two-way ANOVA with Šidák post hoc analysis). Values are means ± SE, n = 6–8 animals.