Table 1.
General kidney cell panel
| Target Cell Type | Clone | Antigen | Fluorophore | Supplier | Concentration | Reference |
|---|---|---|---|---|---|---|
| Endothelial cells | 390 | CD31 | BV605 | Biolegend | 0.4 mg/mL | (23) |
| Pericytes | ME-9F1 | CD146 | BV650 | Miltenyi | 0.4 mg/mL | (24) |
| Fibroblasts and MCs | mEF-SK4 | Feeder cells | PE | Miltenyi | 0.2 mg/mL | (25) |
| Epithelial cells | G8.8 | EpCAM | PerCP-Cy5.5 | Biolegend | 1 mg/mL | (25) |
| SMCs | Duha59 | CD39 | PE-Cy7 | Biolegend | 1 mg/mL | (26) |
| Podocytes | G-8 | Nephrin | AF647 | Bioss | 0.2 mg/mL | (27) |
Conjugated fluorescent antibodies for flow cytometry to identify major cell types of the kidney are shown. The antibody suppliers included BioLegend, Miltenyi Biotech (North Rhine-Westphalia, Germany), and Bioss Antibodies. EpCAM, epithelial cell adhesion molecule; MCs, mural cells; SMCs, smooth muscle cells.