Table 2.

Epithelial cell subtype panel

Target Cell Type	Clone	Antigen	Fluorophore	Supplier	Concentration	Reference
PTCs	FL-1321	LTL	FITC	Vector	0.4 mg/mL	(8)
DCTCs	REA501	EMB	APC	Miltenyi	0.75 mg/mL	(8)
A-ICs	2B8	c-kit	Cy7	Biolegend	0.2 mg/mL	(10)
B-ICs	FL-1321	PNA	Cy5	Vector	0.4 mg/mL	(10)
Principal cells	555	L1-CAM	PE	Miltenyi	1 mg/mL	(10)
Epithelial cells	G8.8	EpCAM	PerCP-Cy5.5	Biolegend	0.2 mg/mL	(28)

Conjugated fluorescent antibodies for flow cytometry to identify epithelial cell subtypes of the kidney are shown. The antibody suppliers included Vector Biolabs (Malvern, PA), Miltenyi Biotech (North Rhine-Westphalia, Germany), and BioLegend. A-ICs, A-intercalating cells; B-ICs, B-intercalating cells; DCTCs, distal convoluted tubule cells; EMB, embigin; EpCAM, epithelial cell adhesion molecule; L1-CAM, L1 cell adhesion molecule; LTL, Lotus tetragonoblus lectin; PNA, peanut agglutinin lectin; PTCs, proximal tubule cells.