Table 3.
Leukocyte subtype fluorescent antibody panel
| Target Cell Type | Clone | Antigen | Fluorophore | Supplier | Concentration | Reference |
|---|---|---|---|---|---|---|
| Leukocytes | 30-F11 | CD45 | AF700 | BioLegend | 1 mg/mL | (21) |
| T cells | 145-2C11 | CD3 | APC | BioLegend | 0.4 mg/mL | (21) |
| Myeloid cells | M1-70 | CD11b | BV421 | BioLegend | 0.5 mg/mL | (21) |
| T helper/T regs | RM4-5 | CD4 | BV605 | BioLegend | 0.4 mg/mL | (21) |
| Cytotoxic T cells | 53-6.7 | CD8 | PerCP-Cy5.5 | BioLegend | 0.2 mg/mL | (21) |
| Macrophages | BM8 | F4/80 | APC-Cy7 | BioLegend | 0.4 mg/mL | (21) |
| Granulocytes | 1A8 | Ly6G | PE-Cy7 | BioLegend | 0.2 mg/mL | (21) |
| Monocytes | HK1.4 | Ly6C | FITC | BioLegend | 0.2 mg/mL | (21) |
| B cells | RA3-6B2 | B220 | PE | BioLegend | 0.2 mg/mL | (21) |
Conjugated fluorescent antibodies for flow cytometry to identify immune cell subtypes of the kidney are shown. The antibody supplier was BioLegend. T regs, T regulatory cells.