Table 4.
Proportion of major cell types in the kidney
| Cell Type |
Protocol 1
|
Protocol 2
|
Protocol 3
|
|||
|---|---|---|---|---|---|---|
| Male | Female | Male | Female | Male | Female | |
| Pericytes | 0.2 ± 0.05 | 0.2 ± 0.3 | 0.2 ± 0.1 | 0.1 ± 0.03 | 0.2 ± 0.04 | 0.2 ± 0.04 |
| Fibroblasts | 0.1 ± 0.03 | 0.2 ± 0.02 | 0.1 ± 0.04 | 0.2 ± 0.05 | 0.09 ± 0.03 | 0.1 ± 0.04 |
| Podocytes | 1.9 ± 0.5 | 3.2 ± 0.9 | 1.3 ± 0.5 | 1.2 ± 0.5 | 4.8 ± 2.2 | 7.0 ± 2.5* |
| Leukocytes | 12.3 ± 2.1 | 13.1 ± 1.9 | 9.4 ± 1.6 | 10.7 ± 2.4 | 13.1 ± 2.5 | 10.4 ± 1.3 |
| Epithelial cells | 1.9 ± 0.7 | 3.8 ± 2.0 | 2.4 ± 0.7 | 4.1 ± 2.2 | 3.1% ± 1.0 | 4.8 ± 2.2 |
| Endothelial cells | 18.6 ± 3.2* | 21.8 ± 3.8* | 11.0 ± 1.9 | 11.3 ± 2.6 | 10.7 ± 1.8 | 8.1 ± 1.6 |
| SMCs | 0.8 ± 0.1 | 1.1 ± 0.2 | 0.5 ± 0.1 | 0.9 ± 0.2 | 0.6 ± 0.1 | 0.6 ± 0.1 |
| Ungated cells | 64.1 ± 5.5 | 56.6 ± 7.6 | 75 ± 4.1 | 71.5 ± 6.6 | 67.4 ± 5.6 | 68.8 ± 6.5 |
Values are means ± SE, n = 8 animals. Percentage of major renal cell types as a proportion of live cells in the kidney is shown. SMCs, smooth muscle cells.
*S
ignificantly (P < 0.05) different between protocols (two-way ANOVA with Šidák post hoc analysis).