Table 6.
Proportion of immune cell subtypes
| Cell Type |
Protocol 1
|
Protocol 2
|
Protocol 3
|
|||
|---|---|---|---|---|---|---|
| Male | Female | Male | Female | Male | Female | |
| Myeloid cells | 66.0 ± 2.8* | 66.7 ± 2.7* | 56.3 ± 2.6 | 53.3 ± 3.0 | 48.1 ± 2.5 | 54.2 ± 2.7 |
| Macrophages | 65.7 ± 3.5 | 70.5 ± 3.6 | 66.5 ± 4.0 | 70.1 ± 2.9 | 62.3 ± 4.8 | 64.6 ± 4.3 |
| Granulocytes | 5.3 ± 1.3# | 1.9 ± 0.2 | 6.9 ± 1.6# | 3.1 ± 1.4 | 6.1 ± 2.2# | 2.2 ± 0.3 |
| Monocyteshi | 5.3 ± 0.9* | 3.0 ± 0.3 | 4.1 ± 0.5 | 2.7 ± 0.2 | 3.2 ± 0.4 | 2.2 ± 0.4 |
| Monocyteslo | 3.5 ± 0.4 | 2.9 ± 0.2 | 4.9 ± 0.4 | 4.6 ± 0.3 | 4.0 ± 0.2 | 3.6 ± 0.3 |
| B cells | 14.1 ± 1.2* | 10.7 ± 0.5* | 3.5 ± 0.7 | 2.6 ± 0.6 | 15.1 ± 1.3* | 13.5 ± 1.4* |
| T cells | 9.1 ± 1.0 | 10.5 ± 0.8 | 18.1 ± 1.3 | 22.6 ± 1.1 | 19.6 ± 3.0 | 19.4 ± 2.0 |
| Cytotoxic | 0.3 ± 0.1 | 0.5 ± 0.2 | 0.3 ± 0.1 | 0.2 ± 0.1 | 24.9 ± 1.8* | 23.5 ± 1.3* |
| T helper cells | 0.8 ± 0.3 | 0.9 ± 0.3 | 50.0 ± 5.2* | 44.7 ± 7.3* | 31.2 ± 4.7* | 44.7 ± 4.8* |
| DN T cells | 94.8 ± 4.1* | 94.7 ± 3.7* | 49.7 ± 5.2 | 54.9 ± 7.3 | 43.7 ± 4.3 | 31.5 ± 5.6 |
| Unstained | 10.8 ± 2.3 | 12.1 ± 3.0 | 22.2 ± 2.9 | 21.6 ± 3.2 | 17.2 ± 2.5 | 12.9 ± 3.3 |
Values are means ± SE, n = 8 animals. Percentage of leukocyte cell subtypes as a proportion of the total amount of immune cells in the kidney is shown. Myeloid and T cell subtypes are expressed as a percentage of the parent population (myeloid cells and T cells). Unstained leukocytes are expressed as a percentage of live leukocytes. DN T cells, double-negative T cells.
*
Significantly (P < 0.05) different between protocols; #significantly (P < 0.05) different between sex (two-way ANOVA with Šidák post hoc analysis).