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Description of the Replication Competent Retrovirus assay. A. Our current assay subjects HEK293 cells are exposed to test articles then passaged for 3 weeks (Amplification Phase). Media from confluent cultures are then filtered (0.45 micron) and evaluated in the S+/L− assay. B. The modified assay tests filtered amplification phase media by Product Enhanced Reverse Transcriptase assay directly and after one week of passage on naïve HEK293 cells (Indicator Phase).
