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. Author manuscript; available in PMC: 2025 Sep 1.
Published in final edited form as: J Virol Methods. 2024 Jun 20;329:114988. doi: 10.1016/j.jviromet.2024.114988

Figure 5.

Figure 5

Transduction of HEK293 and GP4 cells with VSV-G and GALV pseudotyped retroviral vectors. Retroviral vectors pseudotyped with VSV-G and GALV envelopes were standardized by PERT before transducing HEK293 (A and B) and PG4 (C and D) cells. The vectors were diluted at 1:5 (A and C) or 1:20 (B and D) with culture media. Mock groups were transduction using culture media without vectors. Cells were harvested 3 days post-transduction and analyzed by flow cytometry for GFP expression. The results shown were representative of three independent experiments.