Table 3.

Quantitative PCR analysis of amplification phase media for the presence of SV40 T antigen DNA as a surrogate for detecting HEK293T (EOP) End-of-Production Cells. The # designation indicates the replicate flask tested.

Test Article Name	Test Article (SV40 T antigen copies per reaction)	Test Article Spiked with 100 Copies SV40 plasmid (SV40 T antigen copies per reaction)
Water	Undetected	Not tested
Negative Control #1	Undetected	135.2
EOP cells #1	126.145	229.549
EOP cells #12	181.185	311.643
EOP cells #24	227.548	367.154
Supernatant #1	Undetected	133.421
Supernatant #7	Undetected	116.877
Supernatant #13	Undetected	114.256