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. Author manuscript; available in PMC: 2025 Sep 1.
Published in final edited form as: Adv Healthc Mater. 2024 Apr 2;13(22):e2303706. doi: 10.1002/adhm.202303706

Figure 7. Representative histological images and mechanical properties of whole menisci.

Figure 7.

(A) Representative transverse sections with anterior horn (A), central (C), and posterior horn (P) stained with H&E, Safranin O, and Sirius red for fresh, slow-frozen (SF), 2-hour vitrified (2 Hr Vit), and 3-hour vitrified (3 Hr Vit). Magnification: 2×. Scale bars represent 5 mm. Corresponding brightfield images are at 20× with 200 μm scale bars (B-D). The polarized light images, along with their corresponding brightfield counterparts, were captured at 20× with 200 μm scale bars. Equilibrium contact modulus (E) and permeability (F) of the superficial layer in three regions for fresh (N=6; n≥14), SF (N=3; n≥6), 2-hour vitrified (N=3; n≥7), and 3-hour vitrified (N=5; n≥12) menisci, obtained through microindentation tests. N = number of independent menisci; n = number of measurements of all samples in each region. p-value determined with a two-sided t-test. Data presented as the mean ± SD.