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. Author manuscript; available in PMC: 2025 Sep 1.
Published in final edited form as: Allergy. 2024 Jul 20;79(9):2502–2523. doi: 10.1111/all.16231

Figure 3. C5a and C3a receptors play a role in serum+vaccine-induced basophil activation.

Figure 3.

(A) Proportion of C5aR and C3aR on the surface of enriched basophil (black bar) and PBMC (white bar) presented as a percentage or geo mean fluorescence intensity (MFI) value. (B) Effect of C5aR (75 nM) and C3aR (1 μM) antagonist on vaccine induced CD63+CRTh2+ basophil activation in enriched basophil (black bar) and PBMC (white bar). (C) Effect of complement antagonist (C5aRA and C3aRA) on serum+vaccine-induced basophil activation in non-allergics (n=13; blue bar), allergics with low basophil response (n=11; magenta bar) or high basophil response (n=8; green bar). (D) Unbiased clustering analysis FlowSOM showed differences in metaclusters (MC) 9, 10, 11 and 12 in non-allergics and allergics (low or high basophil response) targeted by complement receptor antagonists C5aRA and C3aRA. FlowSOM was performed on concatenated files. (E) Population abundance for MC 9, 10, 11 and 12 in non-allergics or allergics with low or high basophil response following stimulation with serum+vaccine, or in the presence of C5aRA and C3aRA. Data are presented as mean ± SEM. Violin plots are presented with its media represented in black line. Kruskal-Wallis Test, * P<0.05, ** P<0.01, *** P<0.001.