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. Author manuscript; available in PMC: 2025 Sep 1.
Published in final edited form as: Allergy. 2024 Jul 20;79(9):2502–2523. doi: 10.1111/all.16231

Figure 5. Singe cell multiome analyses of individuals with differing basophil responses to COVID vaccines.

Figure 5.

(A) A total of 1 non-reactor, 3 low-(basophil) BAT reactors and 3 high-(basophil) BAT reactors were chosen for single cell ATAC sequencing based on results from the basophil activation tests. The PBMCs were collected from blood and stimulated with autologous patient serum collected after first dose of the vaccine for 20 minutes at 37C before single cell multiome sequencine. (B) Weighted nearest neighbor (WNN) UMAPs of predicted cell types based on azimuth classifications (level 1). UMAP of cells classified from different patient responses. Proportions of predicted cell types for each patient group. (C) RNA transcriptome correlation analysis based on sctransformed normalized data recapitulated across different cell types of stimulation condition. (D) Inferred gene activity correlation analysis based on scATAC-seq data captures similar profiles across treatment conditions.