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. Author manuscript; available in PMC: 2025 Oct 1.
Published in final edited form as: Mol Oral Microbiol. 2024 Mar 4;39(5):354–367. doi: 10.1111/omi.12458

Figure 1. T. denticola produces c-di-AMP during in vitro growth.

Figure 1.

Each T. denticola strain was grown to mid-log phase in NOS prior to harvesting cells. The E. coli laboratory strain BL21(DE3) served as a negative control. Nucleotides were extracted from cell pellets in 80% methanol and c-di-AMP was quantified by LC-MS/MS using an internal standard. As expected, c-di-AMP was not detected (ND) in the E. coli control samples. Data was normalized to total protein. Data are the average with standard deviation for the T. denticola strains (n=5) and duplicate cultures from E. coli. Data was analyzed by ANOVA with Tukey’s post-hoc test.