Figure 5. Ten putative c-di-AMP receptor proteins with the largest difference in PSMs detected between the experimental and control columns.

T. denticola lysate was either passed through an experimental column with c-di-AMP immobilized or a control column with ethanolamine. Columns were extensively washed prior to elution of all proteins with 5 mL of 9M urea. Proteins in duplicate elutions from each column were identified by LC-MS/MS. The total peptide-spectrum matches (PSMs) from the c-di-AMP (blue) and ethanolamine (yellow) columns are shown for the 10 T. denticola proteins with the largest difference in PSMs detected.