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. Author manuscript; available in PMC: 2025 Oct 1.
Published in final edited form as: Mol Oral Microbiol. 2024 Mar 4;39(5):354–367. doi: 10.1111/omi.12458

Figure 6. TDE0087 is a c-di-AMP receptor and osmolarity impacts c-di-AMP levels in T. denticola.

Figure 6.

(A) Recombinant TDE0087 and TDE0294 were tested for binding to biotinylated c-di-AMP. CabP from S. pneumoniae and BSA served as positive and negative controls, respectively. TDE0087 bound c-di-AMP (****p<0.0001) while no significant (ns) binding was observed for TDE0294. Data are representative of at least 3 independent experiments and analyzed by one-way ANOVA with Tukey’s post-hoc test. (B) T. denticola 35405 was grown and placed in a 4:1 mixture of NOS and PBS at various osmotic strengths for 1 hr before harvesting cells for c-di-AMP quantification by LC-MS/MS. Data are the average and standard deviation from c-di-AMP quantified from 5 independent cultures normalized for total protein. Data were analyzed by one-way ANOVA with Tukey’s post-hoc test (****p<0.001, **p<0.01).