Figure 3.

Impulse generation in engrafted cardiomyocytes stimulates the recipient heart

(A) Photography of the experimental setup.

(B) Original recording of an ECG (middle row) and left-ventricular pressure (lower row) under baseline condition and upon photostimulation with blue light in low frequency (470 nm, 3 Hz, upper row).

(C and D) Original recording of an ECG (middle row) and left-ventricular pressure (lower row) under baseline condition and upon photostimulation with high-frequency (C) blue light (470 nm, 6 Hz, upper row) and (D) red light (635 nm, 6 Hz, upper row).

(E) Quantification of pacing capabilities with red light. Nine individual hearts are shown.

(F) Original ECG and left-ventricular pressure recording while adenosine was applied via the coronaries. Pulsed photostimulation with blue light (470 nm, 4 Hz). Photostimulation was initiated after ventricular asystole was established.

(G) Comparison between coupling and cardiomyocyte engraftment. Each data point represents one heart.