Figure 3.

Ras promotes GSC division, not growth

(A and B) Representative GSC images. The red dashed lines outline the GSCs that are biggest in this focal plane. (A, B, D, and E) The images are of the same magnification.

(C) Quantification of GSC sizes. For either genotype, 30 GSCs were quantified. Data represent mean ± SD, and, for either group of data, the mean number is labeled right. Statistical significance was determined by t test. n.s.: p > 0.05.

(D and E) Representative pH3⁺ GSCs. These pH3⁺ GSCs are outlined by yellow dashed lines.

(F) Quantification of GSC mitotic index. The mitotic index was calculated by “pH3⁺/total GSCs ×100%.” Only GSCs within stem cell niches were quantified, and the quantified cell numbers are labeled above. Statistical significance was determined by chi-squared test. ^∗∗∗: p < 0.001. These experiments were performed at 29°C, and 7-day-old flies were used. See all genotypes in Table S1 and the raw quantification data in Table S2. See also Figure S3.