Figure 4.

Ras^V12 overexpression promotes the division of bam mutant GSC-like cells

(A and B) Representative ovarian images. bam^Δ86 is a null, and bam^BG is a strong loss-of-function allele of the bam gene. Both images are of the same magnification.

(C) Quantification of ovarian sizes. For either genotype, 30 ovaries were quantified, and, for each ovary, its biggest 2D size under microscope was used to reflect its 3D size. Data represent mean ± SD, and, for either group of data, the mean number is labeled right. Statistical significance was determined by t test. ^∗∗∗: p < 0.001.

(D and E) Representative germarial images. Vasa-EGFP can outline the germline areas, and pH3 is a mitotic marker. Both images are of the same magnification.

(F) Quantification of germ cell sizes. For either genotype, 30 germaria were scanned by confocal microscope, and, for each germarium, its averaged germ cell size was calculated by “germline area/germ cell numbers.” Data represent mean ± SD, and for the either group of data, the mean number is labeled right. Statistical significance was determined by t test. ^∗∗∗: p < 0.001.

(G) Quantification of the mitotic index. Statistical significance was determined by chi-squared test. ^∗∗∗: p < 0.001. These experiments were performed at 29°C, and 7-day-old flies were used. See all genotypes in Table S1 and the raw quantification data in Table S2. See also Figure S4.