Figure 6.

MAPK signaling is activated specifically in the M phase of mitotic germ cells

(A–B′) Representative images of bam mutant germ cells with nos>Fly-FUCCI. The arrows in (A and A′) indicate a pERK⁺ germ cell that is enlarged in (B and B′). The magenta dashed lines in (B and B′) outline this pERK⁺ germ cell. These experiments were performed at 25°C, and 7-day-old flies were used.

(C–F″) Representative images of mitotic bam mutant germ cells. The prophase, metaphase, anaphase, and telophase were judged by the morphology of condensed chromatids. The yellow dashed lines outline these mitotic germ cells. All images are of the same magnification.

(G–I″) Representative images of wild-type GSCs, cystoblasts, and cystocytes. w¹¹¹⁸ flies were used as the wild type. The yellow dashed lines outline these mitotic germ cells. All images are of the same magnification. These experiments were performed at 29°C, and 7-day-old flies were used. See all genotypes in Table S1. See also Figure S5.