Figure 7.

Ras^V12 overexpression triggers nurse cells to die

(A) A schematic diagram, and nurse cells and the oocyte are highlighted in green.

(B) nos-GAL4-VP14 is activated in nurse cells and oocytes.

(C, E, G, I, and J) Representative ovarian images.

(D) Representative image of a wild-type egg chamber with the nos>GFP genotype.

(F) Representative image of a degrading egg chamber with the nos>Ras^V12 genotype.

(H and K) Quantification of degrading/total egg chambers. Both cycA^C8LR1 and cdk1^B47 are null alleles. 30 ovaries were quantified for each genotype. Data represent mean ± SD, and, for each group of data, the mean number is labeled right. Statistical significance was determined by t test. ^∗∗: p < 0.01; ^∗∗∗: p < 0.001. These experiments were performed at 25°C, and 7-day-old flies were used. See all genotypes in Table S1 and the raw quantification data in Table S2.