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. 2024 Jul 3;19(8):1217–1232. doi: 10.1016/j.stemcr.2024.06.003

Figure 3.

Figure 3

Gains in chromosome 1q are associated with KOSR-free conditions

(A) Timeline of the introduction of popular hPSC culture conditions over the past 25 years beginning with predominantly KOSR-based systems and transitioning into KOSR-free culture regimens. CSCB started using predominantly KOSR-free systems in 2015.

(B) The relative frequency of the most used media in the WiCell dataset over time. The use of KOSR-based media had decreased over time.

(C) The percentage of abnormal karyotypes from the cultures using the most represented media across the WiCell dataset. ∗p = 0.0253; Fisher’s exact test.

(D) The percentage of abnormal karyotypes from the cultures using the most represented matrices across the WiCell dataset. ns, non-significant; Fisher’s exact test.

(E) The percentage of the most common abnormalities in the WiCell dataset across (i) all media (5,650 abnormalities), (ii) KOSR-based media (470 abnormalities), (iii) E8 (1,279 abnormalities), (iv) mTESR (2,295 abnormalities), (v) NutriStem (256 abnormalities), and (vi) StemFlex (391 abnormalities). Less frequent but recurrent aberrations (above 1% of abnormal karyotypes) are indicated in shades of gray. In comparison to KOSR-based medium, the frequency of 1q gain is elevated in E8 (∗∗∗∗p = 7.6 × 10−15), mTESR (∗∗∗∗p = 4.5 × 10−6), NutriStem (∗∗∗∗p = 8.9 × 10−17), and StemFlex (∗∗p = 0.0014); Fisher’s exact test.