Figure 6.

KOSR-based medium, rather than MEFs, diminishes advantage of v1q cells in KOSR/MEF compared to E8/VTN

(A) Schematic representation of experiments aimed at revealing the impact of matrix (VTN or MEFs) versus medium (E8 or KOSR) on the selective advantage of v1q.

(B) Similar numbers of MIFF3 wild-type and v1q hPSCs attach regardless of the medium (E8 and KOSR) or the matrix (VTN and MEFs) at 2 h post-plating. Data shown are the mean ± SD of three independent experiments. ns, non-significant; Unpaired t test.

(C) Cell area at 2 h post-plating is similar between MIFF3 wild-type and v1q hPSCs in each of the conditions tested, indicating similar ability of cell to attach post-plating. Circles represent the cell area of individual cells measured at 2 h post-plating. For each test condition, 30–50 cells were measured across three independent experiments. The line represents the mean of all measurements. ns, non-significant, Mann-Whitney test.

(D) Quantification of the number of phosphorylated focal adhesion kinase (pFAK)-positive focal adhesions. Circles represent the cell area of individual cells measured at 2 h post-plating. For each test condition, 21–32 cells were measured across three independent experiments. The line represents the mean of all measurements. ns, non-significant, Mann-Whitney test.

(E) Representative images of pFAK (green) in MIFF3 wild-type and v1q hPSCs plated in different media (E8 and KOSR) or matrices (VTN and MEFs) at 2 h post-plating. Nuclei are counterstained with Hoechst 33342. Scale bar: 25 μm.

(F) Numbers of MIFF3 v1q hPSCs are higher than wild-type cells in E8/VTN but are not significantly different from wild-type cells in other test conditions at 48 h post-plating. ns, non-significant, ^∗p < 0.05; Unpaired t test. See also Figures S7A and S7B.