Figure 3.

CTSD expression is reduced in day 220 CORD21-ROs lysates

(A and B) WB panel followed by quantitative analyses shows a significant CTSD heavy chain downregulation in CORD21-P1 and -P2 ROs. CD63 and GBA protein expression is significantly reduced in CORD21-P1 and -P2 ROs, respectively.

(C and D) Secreted CTSD heavy chain and active intermediate show no changes in expression levels between CORD21- and control ROs. (B and D) All data were presented as mean +SEM and normalized to the WT sample (n = 3–5 different differentiation experiments each consisting of 48 ROs/sample), ^∗p <0 .05, ^∗∗p <0 .01, ^∗∗∗∗p < 0.0001.