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. 2024 Aug 1;19(8):1189–1204. doi: 10.1016/j.stemcr.2024.07.003

Figure 3.

Figure 3

Functional comparison of E14.5 LSK subsets with ABM counterparts

Comparison of reconstitution of sub-lethally irradiated recipients by E14.5 and ABM iLT-HSCs (A), iST-HSCs (B), iMPP2 (C), iMPP3 (D), and iMPP4 (E) 2 weeks after transplantation. Only values p < 0.05 are shown. ∗, p < 0.05. ∗∗, p < 0.005, ∗∗∗, p < 0.0005, ∗∗∗∗, p < 0.00005. ABM iLT-HSC: n = 8 recipients, 3 donors, 3 experimental days. ABM iST-HSC: n = 8 recipients, 3 donors, 3 experimental days. E14.5 iLT-HSC: n = 5 recipients, donor cells from pools of livers from 2 litters, 2 experimental days. E14.5 iST-HSC: n = 5 recipients, donor cells pools from livers from 2 litters, 2 experimental days. ABM iMPP2: n = 6 recipients, 4 donors, 4 experimental days. ABM iMPP3: n = 5 recipients, 4 donors, 4 experimental days. ABM iMPP4: n = 8 recipients, 4 donors, 4 experimental days. E14.5 iMPP2: n = 6 recipients, donor cells pooled from livers from 4 litters, 4 experimental days. E14.5 iMPP3: n = 6 recipients, donor cells collected from pools of livers collected from 4 litters, 4 experimental days. E14.5 iMPP4: n = 8 recipient mice, donor cells from pools of livers from 4 litters, 4 experimental days. Plt, platelet; Ery, erythroid; Mono, monocyte; B, B cell; T, T cell; NK, natural killer cell; Parentheses, analyzed tissue; PB, peripheral blood; Spl, spleen; Thy, thymus. For (A)–(E), donor-derived reconstitution (Reconstitution %) was determined using donor-specific GFP expression.