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. 2024 Aug 1;19(8):1189–1204. doi: 10.1016/j.stemcr.2024.07.003

Figure 4.

Figure 4

Clonal tracking post-transplantation E14.5 LSK fates

(A) Clonal fate classifications after 2 weeks: (i) Complete multi-outcome, a barcode contributes to all lineages; (ii) Restricted multi-outcome, a barcode contributes to B cells and at least one erythro-myeloid lineage; (iii) Erythro-myeloid restricted, a barcode in erythroblasts, monocytes, and granulocytes; (iv) Myeloid restricted, a barcode in monocytes and granulocytes; (v) Single-lineage restricted, barcode contribution to only one lineage.

(B–F) Heatmaps of barcode reads (i) and histograms summarizing the frequencies of clonal fates (ii) for iLT-HSC (B, n = 41 clones), iST-HSC (C, n = 43 clones), iMPP2 (D, n = 15 clones), iMPP3 (E, n = 57 clones), and iMPP4 (F, n = 168 clones).

(G) Similarity matrix analysis of clonal outcomes.

(H) Bubble plots of the proportion of reads associated with specific clonal outcomes (relative biomass). iLT-HSC: n = 4 recipients, donor cells from pools of 4 litters, 4 experimental days. iST-HSC: n = 3 recipients, donor cells pooled from 2 litters, 2 experimental days. iMPP2: n = 2 recipient, donor cells pooled from two litters, 2 experimental days. iMPP3: n = 2 recipients, donor cells pooled from 2 litters, 2 experimental days. iMPP4: n = 3 recipients, donor cells pooled from 3 litters, 3 experimental days. M, monocyte; N, neutrophil; E, erythroid cell; B, B cell; CPM, counts per million reads.